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. 2021 Mar 5;40:89. doi: 10.1186/s13046-021-01883-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Immunofluorescence staining with CD63 antibody. The images are representative of A549, A549sh/p53 and H1299 cells exposed to different radiation doses. Fluorescent DAPI staining was used to visualize nuclear DNA. The images of the cells were captured by Nikon Eclipse Ti2 confocal microscope with 60x plan apochromatic oil immersion objective lens. Scale bars are 50 μm. Regional foci intensity quantification refers to the evaluation of CD63 expression in A549, A549sh/p53 or H1299 cells exposed to different radiation doses by confocal analysis. The effect of increasing irradiation doses on grand median intensities of nuclear and cytoplasmic foci of DNA:RNA hybrids. Data are presented as grand median ± MAD. (*p < 0.05, **p < 0.01 ***p < 0.001)