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. 2021 Mar 5;40:89. doi: 10.1186/s13046-021-01883-0

Fig. 4.

Fig. 4

Cell irradiation induces the expression of DNA:RNA hybrid structures in NSCLC cell lines. a Immunofluorescence staining with S9.6 antibody. The images are representative of A549, A549sh/p53 and H1299 cells exposed to 10 or 20 Gy. Scale bars are 10 μm. b Regional foci intensity quantification. Effect of increasing irradiation doses on grand median intensities of nuclear and cytoplasmic foci of DNA:RNA hybrids. Data are presented as grand median ± MAD. c Dot blot analysis of RNA extracted from exosome or microvesicle fraction 72 h post-irradiation and immunoblotted with S9.6 antibody. (*p < 0.05, **p < 0.01 ***p < 0.001)