Fig. 7.

Endometriosis upregulated neuronal pNF-κB-p65 in the dorsal root ganglion. a Western blot analyzed the time course of pNF-κB-p65 expression in the DRG from naïve, sham, 3 days, 7 days, 14 days, and 21 days after EM surgery. b Western blot revealed that EM upregulated pNF-κB-p65 protein expression in L3–L5 DRGs. N = 4, one-way ANOVA, *p < 0.05, versus sham and naïve. c–h Immunofluorescence showed neuronal distribution of pNF-κB-p65 in rat DRG of sham and EM 14 days groups. Scale bar 50 μm. i Immunofluorescence staining showed a significantly higher percentage of pNF-κB-p65-immunopositive DRG neurons in EM 14d group compared with that in sham group. n > 100 neurons from 3 rats in each group, *p < 0.05, EM 14d vs sham, chi-square test. j, k Western blot showed that HMGB1, TLR4, MyD88, and pNF-κB-p65 were upregulated in the ipsilateral DRG compared with the contralateral DRG. N = 4, *p < 0.05, Student’s t test, EM 14d-Ipsi versus EM 14d-Contra