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. 2021 Mar 5;18:65. doi: 10.1186/s12974-020-02066-y

Table 1.

List of primary and secondary antibodies used for western blotting analysis

Antibody Host Company Catalog number Dilution Incubation conditions
HMGB1 Rabbit Cell signaling technology #6893 1:1000 Overnight 4 °C
MyD88 Rabbit Abcam ab2064 1:500 Overnight 4 °C
TLR4 Rabbit Invitrogen 48-2300 1:1000 Overnight 4 °C
pNF-κB-p65 Mouse Cell signaling technology #13346 1:1000 Overnight 4 °C
β-actin Mouse Cell signaling technology #3700 1:2000 Overnight 4 °C
Anti-rabbit IgG horseradish peroxidase (HRP) Goat ZSGB-BIO ZDR-5306 1:3000 1 h RT
Anti-mouse IgG horseradish peroxidase (HRP) Goat ZSGB-BIO ZDR-5307 1:3000 1 h RT