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. 2021 Mar 4;20:61. doi: 10.1186/s12934-021-01544-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Physical map of the putative transposable element containing EthRABCD_B2 gene cluster in Rhodococcus sp. B2 and degradation pathway of chloroacetanilide herbicides. a Arrows indicate the sizes, locations, and directions of transcription of the ORFs. Complementation of the EthRABCD_B2-disrupted mutants with different regions is illustrated below the physical map. b The difference of the amino acid sequences of EthB from strain B2 and T3-1. Highlighted characters represent the different residues. c Proposed degradation pathway of pretilachlor, alachlor, acetochlor, propisochlor and butachlor by EthABD_B2. I, pretilachlor. II, Alachlor, R1 = CH₃CH₂-, R2 = CH₃-; Acetochlor, R1 = CH₃-, R2 = CH₃CH₂-; Butachlor, R1 = CH₃CH₂-, R2 = CH₃CH₂CH₂CH₂-: Propisochlor, R1 = CH₃-,R2 = (CH₃)₂CH-.Strain B2 cannot further metabolize three metabolites