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. 2021 Mar 4;12:163. doi: 10.1186/s13287-021-02234-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — GR/HDAC4 mediated the H3K9 deacetylation of TGFβRI induced by high levels of cortisol. a RT-qPCR analysis of HDACs expression in the WJ-MSCs treated with 300, 600, and 1200 nM cortisol after chondrogenic differentiation for 7 days. n = 5. b RT-qPCR analysis of HDAC4 expression in WJ-MSCs treated with cortisol and RU486 (10 μM) after chondrogenic differentiation for 7 days. n = 5. c ChIP-PCR analysis of the H3K9ac level of TGFβRI in WJ-MSCs treated with cortisol, and RU486 (10 μM) or LMK235 (100 nM) after chondrogenic differentiation for 7 days. n = 3. d RT-qPCR analysis of TGFβRI, COL2A1, and ACAN expression in WJ-MSCs treated with cortisol and RU486 (10 μM) or LMK235 (100 nM) after chondrogenic differentiation for 7 days. n = 5. e Western blot analysis of TGFβRI, COL2A1, and ACAN in WJ-MSCs treated with cortisol, RU486 (10 μM), or LMK235 (100 nM) after chondrogenic differentiation for 7 days, n = 5. RT-qPCR, real-time quantitative polymerase chain reaction; GR, glucocorticoid receptor; HDAC4, histone deacetylase 4; H3K9ac, histone 3 lysine 9 acetylation; TGFβRI, transforming growth factor β receptor I; WJ-MSCs, Wharton’s jelly-derived mesenchymal stem cells; ChIP-PCR, chromatin immunoprecipitation-polymerase chain reaction; igG, immunoglobulin G. Data are the mean ± S.E.M. **P < 0.01 vs control