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. 2020 Oct 31;29(3):1120–1137. doi: 10.1016/j.ymthe.2020.10.024

Figure 3.

Figure 3

Knockdown of lncRNA Gm15834 Attenuated Cardiac Hypertrophy

(A) qRT-PCR results of lncRNA Gm15834 knockdown. ∗p < 0.05 versus control group; #p < 0.05 versus Ang-II group. n = 3. (B) Immunofluorescence results of cardiomyocyte areas from in vitro groups. ∗p < 0.05 versus control group; #p < 0.05 versus Ang-II group. n = 3. Scale bar, 50 μm. (C and D) Western blot results of β-MHC (C) and BNP (D) protein expression in lncRNA Gm15834 knockdown groups.. ∗p < 0.05 versus control group; #p < 0.05 versus Ang-II group. n = 6. (E) In vitro transfection of double-labeled LC3 autophagy adenovirus to count autolysosomes and autophagosomes from cardiomyocytes. ∗p < 0.05 versus control group; #p < 0.05 versus Ang-II group. n = 3. Scale bar, 20 μm. (F–H) Western blot results of Beclin-1 (F), LC3-II (G), and p62 (H) protein in lncRNA Gm15834 knockdown groups. ∗p < 0.05 versus control group; #p < 0.05 versus Ang-II group. n = 6.