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. 2020 Nov 5;29(3):1151–1163. doi: 10.1016/j.ymthe.2020.11.004

Figure 2.

Figure 2

Transient Expression Pattern of LPAR4

(A) Immunofluorescence analysis during development at embryonic day 10.5, 12.5, and 16.5 (E10.5, E12.5, and E16.5). Red, CXCR4, RGS5, and LPAR4; green, αSA; DAPI, nuclei. Scale bar, 500 μm. (B) Immunofluorescence (IF) analysis of the correlation between representative cardiac progenitor markers and LPAR4 during differentiation from mouse ESCs and human iPSCs to CMCs. Red, LPAR4; green, αSA; white, Oct4, Nanog, and Nkx2.5; DAPI, nuclei. Scale bar, 20 μm. All experiments were conducted at least in triplicate.