Figure 1.

lnc-MAP3K13-7:1 Is Upregulated in Polycystic Ovary Syndrome (PCOS) and Associated with DNA Methyltransferase 1 (DNMT1) Levels

(A) Quantitative real-time PCR detection of lnc-MAP3K13-7:1 expression levels in ovarian granulosa cells (GCs) from 42 patients with PCOS and 42 normal controls (p = 0.0002). (B) Global DNA methylation of genomic DNA sequences from women with PCOS and normal controls. For each group, the whiskers indicate the 10th and 90th percentiles, the central solid line indicates the median, and the mean for each group is noted by “+” in each box (p < 0.0001). (C and D) Quantitative real-time PCR detection of DNMT1 (C) and DNMT3A (D) expression in the same cohort described in (A) (DNMT1, p = 0.0018; DNMT3A, p = 0.0173). (E) Western blot analysis of DNMT1 protein expression in GCs from 18 patients with PCOS and 18 normal controls (p = 0.0171). The 36 samples were derived from the same experiment. In (A)–(E), data are presented as the mean ± SD. In (A), (C), and (E), ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (Student’s t test). In B, ∗∗∗p < 0.001 (Mann-Whitney U test).