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. 2020 Nov 17;29(3):1279–1293. doi: 10.1016/j.ymthe.2020.11.018

Figure 4.

Figure 4

Expression of Exogenous DNMT1 Rescued lnc-MAP3K13-7:1-Induced Cell Cycle Arrest

(A) Western blot analysis of DNMT1 and proliferation-related marker expression (PCNA, p21, CDK2, p-CDK2, Rb, and p-Rb) normalized to β-actin protein levels after DNMT1 overexpression in KGN cells (n = 3; DNMT1, p = 0.0002; p-Rb, p = 0.002; Rb, p = 0.7602; PCNA, p = 0.0266; p-CDK2, p = 0.0076; CDK2, p = 0.187; p21, p = 0.0207). (B and C) Cell cycle distribution (B) and cell growth curves (C) after exogenous DNMT1 expression in lnc-MAP3K13-7:1-overexpressing cells. The analysis was performed using Duncan’s multiple range test. Different letters (a, b, c, d) in the same column indicate significant differences (p < 0.05; B, n = 3; C, n = 8). (D) Effects of exogenous DNMT1 expression on protein expression in lnc-MAP3K13-7:1-overexpressing cells. DNMT1 overexpression rescued lnc-MAP3K13-7:1 overexpression-induced protein downregulation. The analysis was performed using Duncan’s multiple range test. Different letters (a, b, c, d) in the same column indicate significant differences (p < 0.05, n = 3). In (A)–(D), data are representative of three independent experiments. Mean ± SD. In (A), ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 (Student’s t test). In (B)–(D), p < 0.05 (one-way ANOVA).