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. Author manuscript; available in PMC: 2021 Mar 19.
Published in final edited form as: Mol Cell. 2020 Jan 31;77(6):1340–1349.e6. doi: 10.1016/j.molcel.2020.01.011

Figure 4. The activity of the cytoplasmic RNA exosome on polyadenylated mRNA.

Figure 4.

(A) Schematic representation of MLLFF-Stop-(A)50 mRNA. (B) Time courses of degradation of [32P]cap-MLLFF-Stop-(A)50 mRNA by Dis3L1 and Exo10Dis3L1 at 1.5 mM [Mg2+]. Lane 1 contains RNA markers. (C) Time courses of degradation of [32P]cap-MLLFF-Stop-(A)50 mRNA by Dis3L1 with/without Exo9 at 1.5 mM [Mg2+]. (D) mRNA degradation in ECs assembled on [32P]cap-MLLFF-Stop-(A)50 mRNA and containing MLL-tRNALeu in the P site after 30 min incubation with Dis3L1 in the presence/absence of the Ski complex and Exo9.