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. Author manuscript; available in PMC: 2021 Mar 5.
Published in final edited form as: Cell Rep. 2020 Dec 8;33(10):108476. doi: 10.1016/j.celrep.2020.108476

Figure 6. Contacts between the HalV IRES and Ribosomal Proteins.

Figure 6.

Overview of a cross section of the ribosome showing the 80S-bound HalV IGR IRES in the unrotated state. The exposed section is indicated on the small 80S/HalV IRES schema on the right by a red rectangle.

(A) Blow-up figures on the HalV IRES interaction with the 80S ribosome in the unrotated state.

(B) Interactions between PKI and 18S rRNA in the unrotated state.

(C) View as in (A) for the rotated state. Note the missing interaction between the HalV IRES and uS11 in this state.

(D) View as in (B) for the rotated state. The exposed area is indicated on the small 80S/HalV IRES schema below (B) and (D) by a black circle. IRES nucleotides,which interact with ribosomal proteins and 18S rRNA and which were shown by mutational analysis to be important for IRES function (Figures 3G and 7A), are marked with asterisks. Note that the PK1 region in the rotated state is more flexible than in the unrotated state, as demonstrated by the scanter local densities and resolution compared with the unrotated state. Color coding for all panels: 40S, dark/light yellow; 60S, dark/light blue; HalV, dark/light green; uL1, magenta; uS11, yellow; uS7, red; uL5, teal; uS3, light blue; uS9, purple.