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. 2021 Feb 19;11:624612. doi: 10.3389/fimmu.2020.624612

Figure 5.

Figure 5

Proliferation and GFAP expression after spinal cord injury and TnC fragment application in TnC+/+ and TnC -/- mice. (A) Representative images of Ki67 and TO-PRO-3 immunostainings. More proliferating cells are present at the injury region, whereas a decrease in Ki67+ cells is observed further away from the injury site. Calibration bar for all panels: 50 μm. (B) Proliferation is calculated as the number of Ki67+ nuclei compared to TOPRO-3+ nuclei 7 days after injury. Results are presented as box-and-whisker plots. Even though the two-way ANOVA shows that the treatments’ overall effect was significant (p = 0.043), pairwise comparisons do not show significant differences. n = 3 animals per group for each genotype. (C) GFAP immunoreactivity at the injury region is presented as Integrated density in the box-and-whisker plot. Two-way ANOVA reveals a significant effect of genotype (p < 0.001) and significant interaction of genotype and treatment (p = 0.034). GFAP immunoreactivity is lower at 7 days after injury and application of FnD and Fn(D+A) in TnC-/- versus TnC+/+ mice. n = 3 animals per group for each genotype. (D) Representative images of GFAP and DAPI immunostaining reveal a change in the morphology of GFAP+ astrocytes at the injury region versus more caudal sites. Calibration bar for all panels: 50 μm. (E) GFAP and DAPI immunofluorescence at and around the injury site in the spinal cord of TnC+/+ and TnC-/- mice treated with FnD and Fn(D+A).