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. 2021 Feb 19;11:623726. doi: 10.3389/fimmu.2020.623726

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Copyright © 2021 Sørensen, Park, Gong, Vasanth, Dahle, Korsnes, Phuong, Kiron, Øyen, Pittman and Sørensen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Percentage of head kidney lymphocyte-like cells from Atlantic salmon fed different experimental diets. BG1: Fishmeal + Fish oil diet; BG2: Soybean meal diet; BG3: Fishmeal + Plant oil diet; BG4: Plant ingredients + Fish oil diet; BG5: Plant ingredients + Plant oil diet. (A) Live cells (orange) were separated by excluding the dead cells (yellow); by staining with propidium iodide (PI). (B) Brightfield (BF) area (cell size) vs. side scatter (SSC) intensity (cell internal complexity) plot showing the HK leucocyte population. (C) Percentage of HK lymphocyte-like cells from fish (n=6) fed different experimental diets. Statistically significant differences (p < 0.05) between dietary groups are indicated by different letters. All cell images were captured with 40× objective. Scale bar = 10 µm. BF, brightfield; PI, propidium iodide.