Figure 1.

Ancer-Designed Polytope Neoantigen Constructs Expressed in the SMARRT Platform Generate Polyfunctional CD4 and CD8 T Cell Responses upon Vaccination

(A) Schematic showing polytope design considerations following neoantigen identification. SMARRT replicons expressing neoantigen cassettes designed with Ancer were injected into BALB/c mice at a single dose of 10 μg, and spleens were removed 14 days later and restimulated with a peptide pool containing the top 50 Ancer predicted neoantigens from CT26. T cell function was measured by IFN-γ ELISpot (B), and intracellular cytokine staining is shown for selected replicons (C). Graphs show mean with standard deviation, n = 5 mice per group. Statistical testing was carried out with ordinary one-way ANOVA. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001.