Figure 1.

Design of the Bc-NFκBdODN Conjugate for Targeting B Cell Lymphoma Cells

(A) Predicted hairpin structure of the synthetic Bc-NFκBdODN conjugate. The consensus NF-κB DNA-binding sequence is marked by a red box. (B and C) Mouse A20 lymphoma cells were incubated with 500 nM Cy3-labeled Bc-NFκBdODN or NF-κB dODN for 1–4 h (B) or 4 h (C) and the oligonucleotide uptake (B) or intracellular localization (C) were examined by flow cytometry or confocal microscopy, respectively. Scale bars, 20 μm. (D) Human NHL lymphoma cells were incubated for 4 h in the presence of Cy3-labeled Bc-NFκBdODN at various concentrations. The oligonucleotide uptake was assessed by flow cytometry.