Figure 5.
MAVS Inhibits HBV Replication in Two HBV Tg Mouse Strains
(A) ELISA analysis of HBsAg in serum after hydrodynamic injection and treatment of IFN-α (105 U/kg) and MAVS DNA 20 μg (n = 6). (B) IHC assay of HBsAg and HBx in the liver of HBV mouse (n = 6). Scale bar, 50 μm. (C and D) Continuous ELISA analysis of HBsAg and qPCR analysis of HBV DNA level in serum after AAV injection (n = 8). (E) IHC assay of HBs, HBc, NLRX1, and CASP1 in the liver of HBV mouse. (F) Analysis of HBsAg and HBV DNA levels in serum of the full HBV Tg mouse after AAV-NC or AAV-MAVS injection and treated with or without IFN-α (n = 4). Analysis of the full HBV Tg mouse liver Ifnb1 mRNA level after AAV-NC or AAV-MAVS injection and treated with or without IFN-α (n = 4) is shown. Scale bar, 50 μm. Data represent the mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.