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. 2020 Dec 3;29(3):1335–1348. doi: 10.1016/j.ymthe.2020.11.031

Figure 6.

Figure 6

SP1 Regulates the Expression of MAVS by Interacting with HBc

(A) HepG2 cells were transfected with FLAG-NC, HBx, or HBc plasmid. qPCR analysis of MAVS mRNA expression is shown. (B) HepG2.2.15 cells were transfected with FLAG-NC or HBc plasmid. Western blot analysis and quantification of MAVS protein expression is shown. (C) MAVS mRNA level in liver tissues of the full HBV Tg mouse was detected by qPCR. (D) Detection of the activity of MAVS promoter fragment by dual luciferase reporter gene assay. (E) The relative luciferase activity of promoter-full and promoter-3 was detected after overexpression of HBc and FLAG. (F) After overexpression of HBc and FLAG, the level of MAVS promoter-3 enriched by SP1 was detected. (G) After the overexpression of HBc and SP1 in the HepG2.2.15 cells, they were detected by coIP. Data represent the mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.