Figure 4.

MDEs Transport miR-155-5p and miR-221-5p into Endothelial Cells and Promote Angiogenesis

(A) qPCR indicated the relative miRNA level in M0 and M2 macrophages. (B) Relative level of miRNA in M0 and M2 macrophage-derived exosomes. (C) Relative level of miRNA in MAECs treated with exosomes for 24 h. (D) Schematic illustration of the in vitro co-culture system. (E) Immunofluorescence images show the Cy3-miRNA in MAECs of each group. Scale bars, 30 μm. (F) Average fluorescence intensity of exosomes. (G) Representative micrographs of the transwell assay (original magnification, ×100). The numbers of cells were calculated per high-power field from three independent experiments. (H) Representative micrographs of the 24-h average distance of the wound-healing assay. (I) Representative micrographs of the tube formation assay (original magnification, ×200). The numbers of branch points were calculated by ImageJ. (J–L) Data on transwell, wound-healing, and tube formation assays. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.