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. 2020 Nov 20;29(3):1001–1015. doi: 10.1016/j.ymthe.2020.11.023

Figure 3.

Figure 3

CRISPR-Cas9-Mediated FAH Gene Correction Decreased the Liver and Kidney Damage in HT1 Rabbits

(A and B) Pictures of the livers (A) and kidneys (B) of wild-type, AAV-treated, and AAV-untreated rabbits. (C and D) H&E staining of the liver (C) and kidney (D) sections from the wild-type rabbits, AAV-treated rabbits, and AAV-untreated rabbits. Black squares are amplified in the right images. Scale bars, 100 μm (left panels); 50 μm (right panels). (E) Liver fibrosis was detected by Sirius red staining in the liver tissues of wild-type, AAV-treated, and AAV-untreated HT1 rabbits. Scale bars, 500 μm. (F–H) FAH IHC of the liver sections from the wild-type rabbits (F) and HT1 rabbits injected with AAV8 (G) or PBS (H). The right panel shows a high-magnification view (box with black dashed line). Scale bars: 100 μm (left panels); 50 μm (right panels), respectively. (I) Western blot analysis of the FAH expression in the livers of the wild-type, AAV-treated, and AAV-untreated HT1 rabbits.