Table 1.
Neutralization of variants by mAbs
| Virus | Valuea | mAbs |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| B38 | COVA1-18 | COVA2-15 | DH1041 | DH1042 | DH1043 | DH1047 | H4 | P2B-2F6 | S309 | ||
| D614G | IC50 | 2.10 | 0.0060 | 0.0058 | 0.0094 | 0.011 | 0.0081 | 0.14 | 2.10 | 0.071 | 0.048 |
| IC80 | 12.00 | 0.031 | 0.028 | 0.036 | 0.068 | 0.040 | 0.54 | 18.00 | 1.20 | 0.69 | |
| D614G.B117 | IC50 | 30 (14.2x) | 0.011 (1.8x) | 0.05 (8.7x) | 0.015 (1.6x) | 0.052 (4.7x) | 0.01 (1.3x) | 0.17 (1.2x) | 9.9 (4.7x) | 0.075 (1.1x) | 9.2 (191.4x) |
| IC80 | >50 (> 4x) | 0.16 (5.1x) | 0.95 (34.5x) | 0.039 (1.1x) | 0.13 (1.9x) | 0.05 (1.3x) | 0.64 (1.2x) | >50 (> 3x) | 2.2 (1.8x) | >50 (> 72x) | |
| D614G.N501Y | IC50 | 4.7 (2.2x) | 0.032 (5.2x) | 0.084 (14.6x) | 0.01 (1.1x) | 0.012 (1.1x) | 0.015 (1.8x) | 0.072 (0.5x) | 3.7 (1.7x) | 0.043 (0.6x) | 0.15 (3.1x) |
| IC80 | >50 (> 4x) | 0.19 (6.1x) | 0.93 (33.8x) | 0.034 (1x) | 0.06 (0.9x) | 0.058 (1.5x) | 0.43 (0.8x) | 46 (2.5x) | 0.93 (0.8x) | >50 (> 72x) | |
| D614G.del69-70 | IC50 | 1.5 (0.7x) | 0.0042 (0.7x) | 0.0051 (0.9x) | 0.007 (0.8x) | 0.0095 (0.9x) | 0.0077 (0.9x) | 0.1 (0.7x) | 2.5 (1.2x) | 0.1 (1.4x) | 0.018 (0.4x) |
| IC80 | 9.9 (0.8x) | 0.026 (0.8x) | 0.026 (0.9x) | 0.029 (0.8x) | 0.053 (0.8x) | 0.033 (0.8x) | 0.5 (0.9x) | 26 (1.4x) | 1.1 (0.8x) | 18 (26.8x) | |
| D614G.del69-70.N501Y | IC50 | 2.5 (1.2x) | 0.026 (4.3x) | 0.051 (8.8x) | 0.0044 (0.5x) | 0.0061 (0.6x) | 0.0074 (0.9x) | 0.049 (0.4x) | 3.1 (1.5x) | 0.033 (0.5x) | 0.11 (2.2x) |
| IC80 | 40 (3.3x) | 0.18 (5.8x) | 11.6 (56.4x) | 0.025 (0.7x) | 0.039 (0.6x) | 0.035 (0.9x) | 0.28 (0.5x) | 30 (1.6x) | 1.0 (0.8x) | >50 (> 72x) | |
| D614G.del69-70.Y453F | IC50 | 1.2 (0.5x) | 0.0083 (1.4x) | 0.0055 (0.9x) | 0.0046 (0.5x) | 0.004 (0.4x) | 0.011 (1.4x) | 0.048 (0.3x) | 0.64 (0.3x) | 0.094 (1.3x) | 0.098 (2x) |
| IC80 | 15 (1.3x) | 0.043 (1.4x) | 0.03 (1.1x) | 0.027 (0.8x) | 0.03 (0.5x) | 0.05 (1.3x) | 0.24 (0.4x) | 5.7 (0.3x) | 1.3 (1x) | 11 (15.5x) | |
| D614G.N439K | IC50 | 1.3 (0.6x) | 0.0061 (1x) | 0.011 (1.9x) | 0.0075 (0.8x) | 0.0063 (0.6x) | 0.017 (2.1x) | 0.016 (0.1x) | 3.6 (1.7x) | 0.15 (2.2x) | 0.046 (0.9x) |
| IC80 | 19 (1.6x) | 0.049 (1.6x) | 0.1 (3.8x) | 0.035 (1x) | 0.07 (1x) | 0.059 (1.5x) | 0.33 (0.6x) | 42 (2.3x) | 1.6 (1.3x) | 0.38 (0.6x) | |
Numbers in parentheses followed by “x” represent fold differences over D614G. NA signifies data not available. >3-fold increase in IC50 or IC80 concentration over D614G represents moderate decrease in susceptibility. >10-fold increase in IC50 or IC80 concentration over D614G represents substantial decrease in susceptibility. <0.33-fold of the IC50 or IC80 of D614G represents moderate increase in susceptibility.
a
Unit of IC50 and IC80 concentrations is μg/mL.