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. 2021 Mar 2;9:e10898. doi: 10.7717/peerj.10898

Figure 4. ucOCN inhibited the early differentiation of BMMs.

Figure 4

Experimental design and operation chart (A). RT-qPCR detected osteoclast-relate gene expression (B). TRAP staining (C). RAW264.7 cells were treated with ucOCN for 2 day without inducing factor RANKL, then performed RT-qPCR detection for the mRNA expression (D), Western blot detection for the protein expression (E) of c-Fms and RANK and its gray analysis of protein bands (F). * P < 0.05, ** P < 0.01, n ≥ 3, vs. CN (the ucOCN untreated control group).