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. 2021 Feb 8;6(3):e136841. doi: 10.1172/jci.insight.136841

Figure 7. Reshaping commensal microbiota using B. fragilis reduces GVHD.

Figure 7

Recipient BALB/c mice were treated with a cocktail of broad-spectrum antibiotics for 21 days followed by 2 days of rest. A group of these recipients were administered through oral gavage with either vehicle or live B. fragilis as described in Figure 1. Recipients were monitored for clinical score (A) and survival (B) until 60 days post-BMT (n = 10 per group). Data shown are from 2 combined experiments. (C) Fecal pellets were collected from the recipients on day 30 after BMT and extracted for total DNA, which was used for 16S rRNA sequencing. Data shown are from 1 representative experiment. One-way ANOVA analysis of center log ratio–transformed abundances of major genera (present at >1% in at least 1 sample) is shown (*P < 0.05). (D) PCoA of the Bray-Curtis distances indicates visual and statistically significant separation according to Wd* test (P < 0.007).