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. 2021 Feb 8;6(3):e141776. doi: 10.1172/jci.insight.141776

Figure 5. β1-ICD regulates potassium channel gene expression and potassium currents in cardiac ventricular myocytes.

Figure 5

(A) Experimental design of RNA-Seq experiments from CHL cells stably overexpressing the β1-ICD and from P10 Scn1b WT or Scn1b-null mouse cardiac ventricle. (B) RNA-Seq showed that β1-ICD expression downregulates potassium channel genes, whereas Scn1b-null mice show upregulated potassium channel gene expression in cardiac ventricle. (C) Representative potassium currents recorded from ventricular myocytes obtained from WT and Scn1b-null mice. To assess the I-V relationship, 5-second pulses were applied in +10 mV increments from –70 mV to +70 mV, following a 5-second prepulse to –120 mV from –70 mV holding potential. Scale bars: 5 nA and 2 seconds.