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. 2021 Jan 25;6(2):e134601. doi: 10.1172/jci.insight.134601

Figure 2. Echinatin suppresses multiple agonist–mediated NLRP3 inflammasome activation and assembly.

Figure 2

(A–C) LPS-primed BMDMs were pretreated with echinatin (40 μM) or vehicle and then stimulated with ATP, nigericin, MSU, and poly(I:C); cleaved caspase-1 and production of IL-1β were examined by IB analysis (A); activity of caspase-1 (B) and secretion of IL-1β (C) in SN were assessed. (D) LPS-primed BMDMs were pretreated with indicated dose of echinatin and stimulated with nigericin. IB analysis was used to detect cross-linked ASC in the Triton X-insoluble pellet. (E) IB analysis of cross-linked ASC in the Triton X–insoluble pellet from LPS-primed BMDMs pretreated with echinatin (40 μM) or vehicle and then stimulated with ATP, nigericin, MSU, and poly(I:C). Data are expressed as mean ± SEM (n = 3/group, resulting from 3 independent experiments). Statistics differences were analyzed by unpaired t test (B and C). **P < 0.01, ***P < 0.001.