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. 2021 Jan 25;6(2):e134601. doi: 10.1172/jci.insight.134601

Figure 5. Echinatin inhibits NLRP3 inflammasome activation in vivo and ameliorates LPS-induced septic shock.

Figure 5

(A and B) ELISA of IL-1β (A) and TNF-α (B) in the serum of mice i.p. injected with LPS (20 mg/kg body weight) in the presence or absence of echinatin (20 mg/kg and 40 mg/kg) and MCC950 (20 mg/kg and 40 mg/kg). (Respectively, mock-PBS [n = 8]; mock-LPS [n = 8]; 20 mg/kg echinatin-LPS [n = 8]; 40 mg/kg echinatin-LPS [n = 8]; 20 mg/kg MCC950-LPS [n = 6]; 40 mg/kg MCC950-LPS [n = 6]). (C) Representative FACS plots of neutrophils in the peritoneal cavity from mice i.p. injected with LPS (20 mg/kg body weight) in the presence or absence of echinatin (20 mg/kg and 40 mg/kg). (D) FACS analysis of neutrophil numbers in the peritoneal cavity described in C (n = 6 for each group). (E) Survival of WT mice i.p. injected with 20 mg/kg LPS that pretreated with vehicle (n = 12), echinatin (40 mg/kg, n = 12), MCC950 (40 mg/kg, n = 12), or the combination (n = 12). Data are expressed as mean ± SD. One-way ANOVA, followed by Dunnett’s post-hoc test, was used to assess the differences of multiple groups. *P < 0.05, **P < 0.01, ***P < 0.001 compared with Mock-LPS (A, B, and E) or to echinatin-LPS (D).