Figure 5. CD57⁺ and PD-1⁺ T cells shared a subset of TCRs but differentially expressed features of NK cytotoxicity and T cell activation.

(A) Each segment of the Circos plot represents a TCR junction found in PD-1⁺ (green) or CD57⁺ (gray) cells from the 4 sorted samples. Color bars denote donors. Arcs connect junctions shared between samples. (B) Differentially expressed genes identified by limma analysis contrasting PD-1⁺ (left) and CD57⁺ (right) cells. Genes whose differential expression reached significance are highlighted in blue and red (log_FC > 1; FDR-adjusted P < 0.1). (C) Network shows connected network of genes expressed significantly higher in CD57⁺ than PD-1⁺ cells. Key NKRs are highlighted in red. (D) Selection of enriched pathways in CD57⁺ cells are shown with respective significance of GO term gene overrepresentation in set. (E) Relative expression of pathway-associated genes in CD57⁺ and PD-1⁺ cells, calculated as the mean scaled expression of the gene across all samples. (F) Selection of enriched pathways in the genes differentially expression in PD-1⁺ cells, determined as in D. (G) Relative expression of pathway-associated genes in PD-1⁺ and CD57⁺ cells, analyzed as in E.