Figure 1. CD84 is expressed on immunosuppressive cells in the MM microenvironment.

(A) CD84 expression was analyzed by FACS on MGUS, smoldering disease, and patient-derived CD138⁺CD38⁺ MM cells from the BM. Representative histograms, with percentages of the positively stained cells, are shown (n = 3–10, P = 0.44, 1-way ANOVA). (B) CD138⁺ 5TGM1 cells taken from spleen and BM of 5TGM1-injected C57BL/KaLwRij WT mice. A representative histogram demonstrating the percent of CD84⁺ BM and spleen 5TGM1 cells, is shown (n = 7–8). (C) MM BM stroma cells from MM grown in culture. Representative histograms, showing the percent of CD84⁺ cells, are shown (n = 3–6). (D) Stroma derived from BM aspirates of 5TGM1-injected C57BL/KaLwRij WT or noninjected mice grown in culture. A representative histogram, with percentages of CD84⁺ stroma cells from injected mice is shown (n = 3–7). (E and F) CD14⁺ cells from MM or healthy BM aspirates (E), or MM or healthy PB (F). Representative histograms, indicating percentages of positively stained cells, are shown (n = 7–16).