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. 2021 Feb 22;6(4):e128456. doi: 10.1172/jci.insight.128456

Figure 5. GSC subpopulations vary in their self-renewal potential.

Figure 5

Frequency of clonogenic cells was assessed by extreme limiting dilution analysis using GSC subpopulations derived from (A) TS667, (B) 0308, and (C) MGG8 lines (120, 60, 24, 5, and 1 cells per well; 12–18 replicates per dilution). The experiment was repeated 3 times, and results are shown as mean ± SEM. ANOVA with Tukey’s post hoc tests were used to assess the significance of differences between each GSC subpopulation; *P < 0.05 vs. quadruple-high. GSC, glioblastoma stem cell.