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. 2021 Feb 22;6(4):e134881. doi: 10.1172/jci.insight.134881

Figure 5. The MRCKα downstream pathway is activated upon overexpression of the β1 subunit.

Figure 5

(A) Cells were electroporated with plasmid expressing the rat β1 subunit or pCDNA3 empty plasmid as control at day 3 after isolation. (B) Cells were lysed for Western blot 24 hours later and quantified. Data represent n = 3 biological replicates. Statistical analysis is by Student’s t test, *P < 0.05. (C) At day 1 after isolation, cells were cotransfected with pCMV-tet and pTet-β1 and treated with 20 μM Blebbistatin or DMSO as control. After another 24 hours, 1 μg/mL doxycycline was added to induce gene expression. TEER was measured 24 hours later. ANOVA followed by Bonferroni’s post hoc test was used for statistical analysis. **P < 0.001.