Figure 4.

Minor clonal convergence between CNS immune repertoires. (a) Heatmap depicting clonal overlap between repertoires. Clonal overlap is defined as the number of identical amino acid CDR3 sequences shared between repertoires. BCR and TCR overlap corresponds to the paired CDRH3 + CDRL3/CDRβ3 + CDRα3 amino acid sequence. (b,c) Similarity networks depicting clonal relatedness and expansion between T cell and B cell repertoires, respectively. Size of nodes corresponds to relative clonal expansion. Colour of node corresponds to experimental cohort. Edges between nodes indicate two clones (paired CDRH3 + CDRL3/CDRβ3 + CDRα3 amino acid sequence) that are separated by four or less amino acid mutations. Lower panel highlights those clones containing edges, whereas upper panel depicts all clones. (d) Percentage of unique clones using each V-gene for each experimental cohort. (Online version in colour.)