Figure 3.

The effect of rutin on mitochondrial dynamics in zebrafish larvae. (a) Representative confocal live images of the mitochondria (green fluorescent) with effect of rutin and ethanol on zebrafish larvae muscle. TG(mito:EGFP) zebrafish larvae (4 dpf) were incubated with or without 1 μM rutin under 2% ethanol for 24 h and visualized using a LSM 700 confocal laser microscope (X40). Bar = 10 μm. Quantitative analysis of mitochondrial length in larval muscle cells is presented. Data shown are the means ± SEM of measurements taken from 50 individual cells from three independents experiments. (b) RT-PCR results of mitochondrial fusion and fission related genes affected by rutin following ethanol exposure. (c) Quantitative analysis of relative intensity of drp1, mfn2, and opa1 normalized by gapdh compared to non-treated cells. Data shown are the means ± SEM of measurements taken from three independent experiments. *p < 0.05 and ***p < 0.001.