Figure 5.

The effect of rutin on mitochondrial dynamics in HepG2 cells (a) Representative confocal live images of mitochondria-targeted DsRed showing the effect of rutin. HepG2 cells transfected with mito-DsRed vector were incubated with 1 μM rutin and/or 1% ethanol for 24 h. Bar = 5 μm. Quantitative analysis of mitochondrial length in HepG2 cells. Data shown are the means ± SEM of measurements obtained from 50 individual cells based on three independent experiments. (b–c) Effects of rutin under ethanol exposure on the expressions of DRP1 and MFN2. (b) Quantitative analysis of relative mRNA intensity of DRP1 and MFN2 normalized by GAPDH is presented. (c) Western blot analysis of DRP1 and MFN and quantitative analysis of relative protein intensity of DRP1 and MFN2 normalized by GAPDH are presented Data represents the means ± SEM of measurements obtained from three independent experiments. *p < 0.05, **p < 0.01.