Table 1. List of primers used in this study.
| Primer | Sequence 5’-3’ | Amplicon Size | Ta [°C] | Reference |
|---|---|---|---|---|
| PF1-F | AGAGTTTGATCCTGGCTCAG | 54–60 | [28] | |
| Aa-R | GGCATGCTATTAACACAC | 469 bp | 54 | This study |
| (combined with PF1) | ||||
| Fn-R | GTCATCGTGCACACAGAATTGCTG | 360 bp | 60 | [29] |
| (combined with PF1) | ||||
| Pi-R | GTTGCGTGCACTCAAGTCCGCC | 660 bp | 56 | [30] |
| (combined with PF1) | ||||
| Pg-R | CAATACTCGTATCGCCCGTTATTC | 478 bp | 59 | [31] |
| (combined with PF1) | ||||
| SDent-16S-F | TGAAGGAGGAGCTTGCTTCTC | 59 | [15] | |
| SDent-16S-R | CAAACAGTTATCATGCAATAACTG | 137 bp | 59 | [15] |
| Ssal-M18-C4-F2 | GAGGTCCGGTTAATGGTTGT | 54 | This study | |
| Ssal-M18-C4-R | CTATGCTGGAGATGACGG | 252 bp | 54 | This study |
Ta = annealing temperature of primer.