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. 2021 Feb 23;17(2):e1009404. doi: 10.1371/journal.pgen.1009404

Fig 4. Gene expression in wild-type and pearl-eye pigeons.

Fig 4

(A) Volcano plot with results from differential expression analysis of iris of the two phenotypes, comparing log-fold changes in expression with FDR-adjusted P-values. Significant results are highlighted in blue and SLC2A11B is indicated. (B) Differences in expression of SLC2A11B in the iris of both phenotypes as measured by RT-qPCR. Expression levels are normalized to the expression of beta-actin (ACTB). (C) Allele imbalance for the candidate causal mutation (AKCR02000030.1:1,895,934bp) in three pigeons heterozygous for the wild-type (G) and pearl-eye (A) alleles. The bars indicate for each individual the proportion of reads supporting each allele.