Fig 8.
Current-voltage relationship of CaV2.2 in the presence and absence of 20 nM CVID from HEK 293T cells transfected by (A) calcium phosphate, (C) FuGENE and (E) Lipofectamine 3000. CaV2.2-mediated currents were evoked by depolarization steps from a holding potential of –100 mV followed by –60 mV to +50 mV in 5-mV increment. Current-voltage relationship of NaV1.7 in the presence and absence of 200 nM TTX obtained from HEK 293T cells transfected by (B) calcium phosphate, (D) FuGENE and (F) Lipofectamine 3000. NaV1.7-mediated currents were evoked by step depolarization from a holding potential of –80 mV followed by –60 mV to +55 mV in 5-mV increment. Data are presented as mean ± SEM, with N = 4–9 cells per data point.