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. Author manuscript; available in PMC: 2021 May 19.
Published in final edited form as: J Vis Exp. 2020 Nov 19;(165):10.3791/61912. doi: 10.3791/61912

Figure 2: Titration of in-house conjugated ligand panel antibodies.

Figure 2:

Titrations of in-house conjugated antibodies were performed on PBMCs from a healthy donor using five different concentrations: 0.625, 1.25, 2.5, 5, and 10 μg/mL. Titers for anti-HLA-DR, anti-ICAM-1, anti-CCR2, anti-CD14, anti-CD11b, and anti-LFA-3 were determined by gating on CD3−CD7− cells. Titers for anti-CD3, anti-pan HLA class I, anti-CD7, anti-CD48, anti-LLT-1, anti-HLA-C,E, anti-HLA-E, anti-FasR, anti-Nectin-1, anti-MICA/MICB, anti-DR4/DR5, anti-ULBP-1,2,5,6, anti-Nectin-2, anti-CD155, anti-HLA-Bw4, anti-HLA-Bw6, anti-CD33, anti-CD56, and anti-B7-H6 were determined by gating on live cells. Titers for anti-CD4 and anti-CD8 were determined by gating on CD3+ cells. The red arrows indicate the titer selected for each antibody.