Figure 3: NK panel gating strategy and performance.

(A) Serial negative gating from whole PBMCs to NK cells is shown in a healthy donor. Intact, bead and event-length gates ensure successful gating to single cells. Cisplatin staining was performed as a Live/Dead stain. T cells and B cells were excluded using CD3 and CD19. Monocytes were excluded by negative gating on CD4 and CD14/CD33 and by further negative gating of CD56-/HLA-DR^bright cells. CD56 and CD16 were used to identify different subsets of NK cells (CD56^bright, CD56^dim and CD56⁻). (B) Examples of expression of NK cell receptors on NK cells from one healthy donor purified by magnetic-bead isolation.