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. Author manuscript; available in PMC: 2021 May 19.
Published in final edited form as: J Vis Exp. 2020 Nov 19;(165):10.3791/61912. doi: 10.3791/61912

Figure 4: Ligand panel gating and performance.

Figure 4:

(A) Gating of major immune cell subsets from PBMCs derived from a healthy donor following normalization, calibration bead removal, and debarcoding. (B) Expression of ligands for NK cell receptors as well as several myeloid markers on live PBMCs. Staining for all ligands except Nectin-1 and B7-H6 is shown on PBMCs from acute dengue patients. Staining for Nectin-1 and B7-H6 is shown on PBMCs from HIV-infected individuals who were virologically suppressed.