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. Author manuscript; available in PMC: 2021 May 19.
Published in final edited form as: J Vis Exp. 2020 Nov 19;(165):10.3791/61912. doi: 10.3791/61912

Table 1: NK panel.

Markers are ordered according to the isotopic mass of the metal to which they were conjugated. 191Ir/193Ir is the natural abundance of the nucleic acid intercalator. 194Pt/195Pt is the natural abundance of cisplatin.

Specificity Clone Isotope Purpose Surface/Intracellular
CD57 HCD57 89Y Maturity/Memory surface
CD45 HI30 102Pd, 104Pd, 106Pd, 108Pd Barcoding surface
HLA-DR Tü36 Qdot 655 (112Cd-114Cd) Activation/Lineage surface
CD3 UCHT 115In T cell lineage surface
CD38 HIT2 141Pr Activation Marker surface
CD69 FN50 142Nd Activation Marker surface
CD33 WM53 143Nd Monocyte lineage surface
CD14 M5E5 143Nd Myeloid lineage surface
CD2 RPA-2.10 144Nd Activation/Maturity surface
LILRB1 MAB20172 145Nd Inhibitory Receptor surface
CD19 HIB19 146Nd B cell lineage surface
CD8 SK1 147Sm T cell lineage and NK cell Activation/Maturity surface
FcRg polyclonal 148Nd Maturity/Adaptive intracellular
CD4 SK3 149Sm T cell lineage surface
Syk 4D10.2 150Nd Signaling intracellular
CD62L DREG-56 151Eu Activation surface
ki-67 Ki-67 152Sm Proliferation intracellular
KIR2DS4 179315 153Eu Activating Receptor surface
NKp46 9.00E+02 155Gd Activating Receptor surface
NKG2D 1D11 156Gd Activating Receptor surface
TIGIT 741182 157Gd Inhibitory Receptor surface
2B4 C1.7 158Gd Activating Receptor surface
DNAM-1 DX11 159Tb Activating Receptor surface
FAS-L NOK-1 160Gd Apoptosis surface
NKp30 P30-15 161Dy Activating Receptor surface
Siglec-7 S7.7 162Dy Inhibitory Receptor surface
NKG2C 134522 163Dy Maturity/Memory surface
NKp44 P44-8 164Dy Activating Receptor surface
CD96 NK92.39 165Ho NKG2 Co-receptor surface
KIR2DL1/KIR2DS5 143211 166Er Inhibitory Receptor surface
CD94 DX22 167Er Activating Receptor surface
CXCR6 K041E5 168Er Memory surface
PD1 EH12.2H7 169Tm Inhibitory Receptor surface
KIR2DL5 UP-R1 170Er Inhibitory Receptor surface
NKG2A 131411 171Yb Inhibitory Receptor surface
NTB-A NT-7 172Tb Activating Receptor surface
KIR3DL1 DX-9 173Yb Inhibitory Receptor surface
CD56 NCAM16.2 174Yb NK cell lineage surface
KIR2DL3 180701 175Lu Inhibitory Receptor surface
Perforin B-D48 176Yb Cytolytic Protein intracellular
DNA-1/DNA-2 NA 191Ir/193Ir Nucleated cells surface
Cisplatin NA 194Pt/195Pt Viability surface
CD16 3G8 209Bi FcgRIII receptor surface