. Author manuscript; available in PMC: 2021 May 19.

Published in final edited form as: J Vis Exp. 2020 Nov 19;(165):10.3791/61912. doi: 10.3791/61912

Table 2: Ligand panel.

Markers are ordered according to the isotopic mass of the metal to which they were conjugated. ¹⁹¹Ir/¹⁹³Ir is the natural abundance of the nucleic acid intercalator. ¹⁹⁴Pt/¹⁹⁵Pt is the natural abundance of cisplatin.

Specificity	Clone	Isotope	Purpose	Surface/Intracellular
HLA-DR	L243	⁸⁹Y	Antigen presenting cells, activation marker	surface
CD45	HI30	¹⁰²Pd, ¹⁰⁴Pd, ¹⁰⁶Pd, ¹⁰⁸Pd	Barcoding	surface
CD19	SJ25-C1	Qdot 655 (¹¹²Cd-¹¹⁴Cd)	Lineage	surface
CD3	UCHT1	¹¹⁵In	Lineage	surface
Pan HLA class I	W6/32	¹⁴³Nd	KIR ligands	surface
CD7	CD7-6B7	¹⁴⁴Nd	Lineage	surface
CD8	SK1	¹⁴⁵Nd	Lineage	surface
CD48	BJ40	¹⁴⁶Nd	2B4 ligand	surface
ICAM-1	HA58	¹⁴⁸Nd	LFA-1 ligand	surface
LLT-1	402659	¹⁴⁹Sm	CD161 ligand	surface
CD4	OKT4	¹⁵¹Eu	Lineage	surface
HLA-C,E	DT9	¹⁵³Eu	KIR ligands	surface
CCR2	K036C2	¹⁵⁴Sm	Monocyte functional marker	surface
HLA-E	3D12	¹⁵⁵Gd	NKG2A/CD94 and NKG2C/CD94 ligand	surface
Fas (CD95)	DX2	¹⁵⁶Gd	FasL receptor	surface
Nectin-1	R1.302	¹⁵⁷Gd	CD96 ligand	surface
MICA/B	159227/236511	¹⁵⁸Gd	NKG2D ligands	surface
DR4/5	DJR1/DJR2-2	¹⁵⁹Tb	TRAIL receptors	surface
ULBP-1/2,5,6	170818/165903	¹⁶¹Dy	NKG2D ligands	surface
Nectin-2	TX31	¹⁶⁴Dy	DNAM-1, TIGIT, and CD96 ligand	surface
CD155	SKII.4	¹⁶⁵Ho	DNAM-1, TIGIT, and CD96 ligand	surface
HLA-Bw4	REA274	¹⁶⁶Er	KIR3DL1 ligand	surface
HLA-Bw6	REA143	¹⁶⁸Er	KIR null allele	surface
CD14	M5E2	¹⁶⁹Tm	Lineage	surface
CD11b	ICRF44	¹⁷⁰Er	Lineage	surface
LFA-3	TS2/9	¹⁷¹Yb	CD2 ligand	surface
CD33	WM53	¹⁷²Yb	Lineage	surface
CD56	NCAM16.2	¹⁷⁴Yb	Lineage	surface
B7-H6	875001	¹⁷⁶Yb	NKp30 ligand	surface
DNA-1/DNA-2	NA	¹⁹¹Ir/¹⁹³Ir	Nucleated cells	surface
Cisplatin	NA	¹⁹⁴Pt/¹⁹⁵Pt	Viability	surface
CD16	3G8	²⁰⁹Bi	Lineage	surface