Skip to main content
. Author manuscript; available in PMC: 2022 Feb 18.
Published in final edited form as: Mol Cell. 2021 Jan 29;81(4):649–658. doi: 10.1016/j.molcel.2021.01.012

Figure 2. Mechanisms of repriming and ssDNA gap formation in different organisms.

Figure 2.

In E. coli, the DnaG primase, as part of the PriC system, interacts with DnaB and promotes repriming in both the leading and lagging strand (left). In budding yeast, repriming is promoted by the Polα/Primase complex and Ctf4, a replisome factor that bridges the MCM component of the CMG helicase and the Polα/Primase complex (middle). In vertebrates, repriming is ensured by PRIMPOL. How recoupling of leading strand synthesis and the CMG helicase occurs after PRIMPOL-mediated repriming in vivo is unknown.