Skip to main content
. Author manuscript; available in PMC: 2021 Mar 5.
Published in final edited form as: Neuron. 2019 Jun 20;103(4):673–685.e5. doi: 10.1016/j.neuron.2019.05.037

Figure 5: Dendritic spines are morphologically immature in Scn2a+/− cells.

Figure 5:

A: Examples of dendritic morphology in P22–25 Scn2a+/+ (black) and Scn2a+/− (cyan) pyramidal cells.

B: Overall dendrite length and branch points in apical and basal trees. Open circles are single cells. Box plots are median, quartiles, and 90% tails. (Scn2a+/+: n = 10 cells; Scn2a+/−: n = 10 cells). No differences across genotype noted.

C: Examples of spines along apical dendrites of immature (P5–6) and more mature (P24–36) neurons.

D: Overall spine length from shaft to spine head, volume of spine head relative to total volume of head and shaft, and number of spines per length of dendrite were measured in apical dendrites of P5–6 neurons (n = 560–685 spines per group, 1–4 dendritic branches per cells, 10 cells per group; note that basal dendrites often lacked spines at this age and were therefore not assessed). Spines along both apical and basal dendrites were imaged in P24–36 neurons (n = 500–600 spines per group, 1–3 dendritic branches per cell, 6 cells per group). *: p < 0.05, Mann-Whitney.