Figure 7: Scn2a haploinsufficiency impairs synaptic plasticity.
A: Recording configuration for burst-based LTP experiments. Stimulating electrode was placed in layer 1 and synaptic stimulation was paired with somatic current-evoked AP bursts.
B: Example of EPSPs before and after LTP pairing protocol. Lighter shades are post-induction data (15–25 minutes after pairing). Stimulus artifact removed for clarity.
C: EPSP slope (first 2 ms), membrane potential (Vm) and input resistance (Rin) vs time before and after LTP induction. Circles and bars are mean ± SEM.
D: EPSC slope per cell in Scn2a+/+ cells with and without TTX and Scn2a+/− cells. *: p = 0.0002, Kruskal-Wallis test; WT vs Scn2a+/−: p = 0.0007, WT vs TTX: p = 0.002, Dunn’s multiple comparisons test.