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. 2021 Mar 5;10:e60779. doi: 10.7554/eLife.60779

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© 2021, Norris et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 3. — (A) Illustration of injection of retroAAV-DIO-tdTomato in POA and AAV5-DIO-eYFP in a Pdyn-Cre mouse. (B) Diagram of POA region depicted in (C) showing antero- (green) and retrograde (red) labeling of Pdyn+ neurons in POA. (D) Diagram of parabrachial nucleus (PBN) region depicted in (E) showing retrograde labeling from POA (red) and eYFP expression (green). Yellow cells in overlay image, marked with arrow heads, illustrate dual labeling by locally injected and retrograde viruses. (F) Illustration of injection of retroAAV-DIO-tdTomato in POA and AAV5-DIO-eYFP in an Penk-Cre mouse. (G) Diagram of POA region depicted in (H) show antero- (green) and retrograde (red) labeling of Penk+ neurons in POA. (I) Diagram of PBN region depicted in (J) showing retrograde labeling from POA (red) and eYFP expression (green). Yellow cells in overlay image, marked with arrow heads, illustrate dual labeling by locally injected and retrograde viruses.