Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Alexa Fluor 633 goat polyclonal anti-rabbit IgG | Invitrogen | Cat# A-21070, RRID:AB_2535731 | 1:1000 |
| Antibody | Phospho-c-Fos (Ser32) Rabbit monoclonal | Cell Signaling Technology | Cat# 5348S RRID:AB_10557109 |
1:500 |
| Chemical compound, drug | Clozapine N-oxide dihydrochloride | Hellobio | Cat# HB6149 | |
| Chemical compound, drug | Naloxone hydrochloride | Tocris | Cat# 0599 | |
| Chemical compound, drug | Naltrexone hydrochloride | Sigma-Aldrich | Cat# N3136 | |
| Chemical compound, drug | Norbinaltorphimine (norBN) | Sigma-Aldrich | Cat# N1771 | |
| Chemical compound, drug | U50,488 (U50) | Sigma-Aldrich | Cat# D8040 | |
| Strain, strain background (Mus musculus) | Pdyntm1.1(Cre)Mjkr | Gift from Brad Lowell, Harvard | RRID:MGI:5562890 | |
| Strain, strain background (Mus musculus) | B6;129S-Penktm2(Cre)Hze/J | The Jackson Laboratory | RRID: IMSR_JAX: 025112 | |
| Strain, strain background (Mus musculus) | Slc17a6tm2(cre)Lowl/J | Gift from Brad Lowell, Harvard | RRID: IMSR_JAX: 028863 | |
| Strain, strain background (Mus musculus) | B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | The Jackson Laboratory | RRID: IMSR_JAX: 007914 | |
| Strain, strain background (Mus musculus) | C57BL/6J | The Jackson Laboratory | RRID: IMSR_JAX:000664 | |
| Strain, strain background (AAV5) | AAV5-EF1a-DIO-hChR2(H134R)-EYFP | Washington University Hope Center Viral Vector Core | N/A | (2.5 × 1013 vg/ml) |
| Strain, strain background (AAV5) | AAV5-hSyn-DIO-hM4D(Gi)-mCherry | Addgene | Addgene_44362-AAV5 | (7 × 10¹² vg/ml) |
| Strain, strain background (rAAV2-retro) | AAV2-retro-DIO-ChR2-eYFP | Washington University Hope Center Viral Vector Core | N/A | (2.8 × 1012 vg/ml) |
| Strain, strain background (AAV) | AAV-retro-CAG-FLEX-tdTomato-WPRE | Addgene | Addgene_51503-AAVrg | (1 × 1013 vg/ml) |
| Strain, strain background (AAV2) | AAV2-retro-DO_DIO-tdTomato_EGFP-WPRE-pA | Addgene | RRID:Addgene_37120 | (8 × 10^12 GC/ml) |
| Strain, strain background (AAV5) | AAV5-Ef1a-DIO-eYFP | Washington University Hope Center Viral Vector Core | N/A | (1.4 × 1013 vg/ml) |
| Strain, strain background (rAAV2-retro) | AAV2-retro-GFP-Cre | Washington University Hope Center Viral Vector Core | N/A | (3 × 1013 vg/ml) |
| Strain, strain background (AAV5) | AAV5/hSyn-dio-hm4D(Gi)-mcherry | Addgene | RRID:Addgene_44362 | (7.8 × 10^12 vg/ml) |
| Sequence-based reagent | RNAscope probe Pdyn | Advanced Cell Diagnostics | accession number NM_018863.3 | probe region 33–700 |
| Sequence-based reagent | RNAscope probe Penk | Advanced Cell Diagnostics | accession number NM_001002927.2 | probe region 106–1332 |
| Sequence-based reagent | RNAscope probe Slc17a6 | Advanced Cell Diagnostics | accession number NM_080853.3 | probe region 1986–2998 |
| Sequence-based reagent | RNAscope probe GFP | Advanced Cell Diagnostics | accession numberAF275953.1 | probe region 12–686 |
| Sequence-based reagent | RNAscope probe Cre | Advanced Cell Diagnostics | accession number KC845567.1 | probe region 1058–2032 |
| Sequence-based reagent | RNAscope probe Cck | Advanced Cell Diagnostics | accession number NM_031161.3 | probe region 23–679 |
| Software, algorithm | ImageJ | NIH | RRID: SCR_003070 | |
| Software, algorithm | ResearchIR | FLIR Systems Inc | N/A | |
| Software, algorithm | Leica Application Suite Advanced Fluorescence | Leica Microsystems | N/A | |
| Software, algorithm | Bonsai | Bonsai-rx.org | N/A | |
| Software, algorithm | DeepLabCut | Mathis Lab (Mathis et al., 2018) | N/A |