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. 2021 Feb 18;9:622198. doi: 10.3389/fcell.2021.622198

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Copyright © 2021 Lu, Che, Qu, Zheng, Yang, Bao, Li, Wang, Jin, Wang, Xiao, Qi and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the correlation between succinylation regulators and m6A regulators at protein level. (A) The expression pattern of CPT1A, KAT2A, SIRT5, SIRT7, LRPPRC, and EIF3B between ccRCC tissues and corresponding normal tissues by immunohistochemistry. Error bars show standard error of the mean, and the middle bar represents the median expression level of each molecule. (B) The correlation analysis between CPT1A and LRPPRC, SIRT5 and EIF3B, CPT1A and EIF3B, SIRT5 and LRPPRC according to immunohistochemistry scores in 42 ccRCC tissues. (C) In ACHN cells, the expression of LRPPRC and EIF3B were detected by western blotting after transient knockdown of CPT1A and SIRT5, respectively.