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Primers and probes used in this study.
| Organisms | Target gene | Sequence (5′–3′) | Cycling parameters |
|---|---|---|---|
| SARS-CoV-2 | Probe & Primer ORF1a/b |
FACAGGTGGAACCTCATCAGGAGATGC-BBQ F-GTGARATGGTCATGTGTGGCGG R-CARATGTTAAASACACTATTAGCATA |
55 °C 10′ 94 °C 3′ 94 °C 15″ 58 °C 30″ 45x. |
| Primer &Probe N gene |
F-AAATTTTGGGGACCAGGAAC R-TGGCAGCTGTGTAGGTCAA PFAM-ATGTCGCGCATTGGCATGGA-BHQ |
55 °C 10′ 94 °C 3′ 94 °C 15″ 58 °C 30″ 45x. |
