Skip to main content
. Author manuscript; available in PMC: 2022 Mar 4.
Published in final edited form as: Mol Cell. 2021 Jan 15;81(5):1074–1083.e5. doi: 10.1016/j.molcel.2020.12.036

Figure 3. RADX prevents RAD51 mediated strand-exchange and D-loop formation.

Figure 3.

(A) Schematic of RAD51-mediated strand exchange assay (jm, joint molecules; nc, nicked circular dsDNA). RADX was added either 10 minutes prior to the dsDNA in panels B and F or at the same time as dsDNA in panel C. (B) A representative gel and quantitation from n=3 strand exchange assays are shown. Error bars are SD. (C) Strand exchange assay in which RADX or RADX OB2m was added at the same time as the dsDNA. Representative gel and quantitation from n=3 experiments. (D) Schematic of D-loop formation assay. (E) A representative gel of the D-loop assay and quantitation (n=3, +/− SD). (F) Strand exchange assay with BRC3/4 and/or RADX proteins added at the same time as RPA.