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. Author manuscript; available in PMC: 2022 Mar 4.
Published in final edited form as: Mol Cell. 2021 Jan 15;81(5):1074–1083.e5. doi: 10.1016/j.molcel.2020.12.036

Figure 6. RADX interacts with RAD51 to maintain replication fork stability.

Figure 6.

(A) Immunoblots of U2OS, or RADXΔ cells infected with lentivirus expressing the wild-type (WT) RADX or RADX QVPK (EV = empty vector). The passage number after infection and selection is indicated. (B) Cells were imaged for γH2AX. P values were derived from a one-way ANOVA with Dunnett’s multiple comparison test. (C) Cells were labeled with CldU and IdU as indicated and then analyzed by DNA combing. Representative images and experiment are shown with P values derived from a one-way ANOVA with Dunnett’s multiple comparison test. (D) Fork symmetry was assessed as indicated. At least 177 DNA fibers were measured from four biological replicates. P values were derived from a Kruskal-Wallis test. (E) The percentage of DR-GFP-positive U2OS cells after overexpression of empty vector, RADX WT or RADX QVPK and I-Scel expression vector. Immunoblots show the level of overexpression of RADX WT and RADX QVPK (Mean+/−SEM, n=3 in which 25,000 cells were scored per experiment; p-value derived from One-way ANOVA with Dunnett’s multiple comparisons test).